关键词：蓝花楹；实生苗茎尖外植体；组织培养技术 [gap=920]Key words：Jacaranda acutifolia；explant of seedling stem tip；tissue culture technique
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Plant tissue culture technique 植物组织培养技术
modified tissue culture technique 改良组织块法
technique tissue culture 组织培养技术
technique of tissue culture 组织培养技术
Objective To explore the feasibility of tissue engineered articular cartilage constructed by the technique of centrifuge tube culture and study its structure and function.
目的探索应用离心管培养技术进行体外构建组织工程化关节软骨组织的可行性，并研究其结构和功能。
METHODS: Cell culture technique and tissue engineering method were used.
方法：采用细胞培养技术和组织工程学方法。
In this paper the detailed procedures for slice culture technique of brain tissue were introduced, including the equipments required, reagents, preparation of brain tissue slice and incubation.
本文对脑组织切片培养技术进行了详细的介绍，包括仪器设备、培养液的配制、脑组织切片制备及培养过程等。
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